T-DNA insertion mutagenesis in Arabidopsis: mutational spectrum
نویسندگان
چکیده
منابع مشابه
Characterization of T-DNA insertion sites in Arabidopsis thaliana and the implications for saturation mutagenesis.
A key component of a sound functional genomics infrastructure is the availability of a knockout mutant for every gene in the genome. A fruitful approach to systematically knockingout genes in the plant Arabidopsis thaliana has been the use of transferred-DNA (T-DNA) from Agrobacterium tumefaciens as an insertional mutagen. One of the assumptions underlying the use of T-DNA as a mutagen is that ...
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With the availability of complete genome sequences of several organisms, the focus has shifted from structural genomics to functional genomics, specifically in plants where the complete genomic sequences are becoming available i.e., Arabidoposis and rice. Agrobacterium mediated transformation which is exploited for transgenic technology is also being used as an effective mutagen and as a tool f...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 1991
ISSN: 0960-7412,1365-313X
DOI: 10.1111/j.1365-313x.1991.00071.x